There is a report that an exercise performance changes by the wearing graduated compression stockings. It is reported that a raising angle of the gastrocnemius muscle enthesis enlarged by a graduated compression stockings wearing. The aim of this study was to examine morphological change at the triceps muscle of calf during the application of a below-knee graduated compression stocking. Ten healthy males (20.8±0.8 years) volunteered to this study. The condition assumed ①Control (bare foot), ②Normal (Normal stocking ), ③ES (graduated compression stocking). Thickness and angle of pennation at the triceps muscle of calf were taken by ultrasound scanning using NOBLUS (Hitachi Medico Co., Ltd.) with relaxed standing position. In addition, the wearing pressure and the leg greatest diameter were measured. ES was significantly high in the wearing pressure in comparison with Normal. Significant difference was not seen in the pennation angle of the gastrocnemius muscle medial head between conditions. The depth from a deep aponeurosis to skin significantly had a long ES from in comparison with Control, Normal. Therefore, these results suggested that the graduated compression stocking prevented muscle and subcutaneous fat of the calf hanging down for the gravity.